Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/25

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Current revision (20:27, 25 October 2013) (view source)
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Because the gel from Wednesday looked weird, I recut J06702 with just EcoRI or with E and X. I ran them on a gel along with uncut plasmid<br>
Because the gel from Wednesday looked weird, I recut J06702 with just EcoRI or with E and X. I ran them on a gel along with uncut plasmid<br>
[[image:13.10.25_cropped.png‎||J06702 digest]]
[[image:13.10.25_cropped.png‎||J06702 digest]]
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The band should be 3024bp but it's closer to 4kb. I don't know what happened. I will try the ligation and then sequence. I'll also send the original miniprep off for sequencing.
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Because the gel from Wednesday looked weird, I recut J06702 with just EcoRI or with E and X. I ran them on a gel along with uncut plasmid
J06702 digest
The band should be 3024bp but it's closer to 4kb. I don't know what happened. I will try the ligation and then sequence. I'll also send the original miniprep off for sequencing.


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