Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/13

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(Autocreate 2014/02/13 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (16:52, 14 February 2014) (view source)
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==02/13/2014==
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Golden gate colonies
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*1-4 did not grow
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**Realized the Lux boxes were not correct. Ordered new ones
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*5-6 grew >10 small colonies
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**Picked two from each plate into 3mL LB+amp
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*Positive control grew hundreds of medium sized colonies
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Got some more K12s from Kylie to make competent cells. Picked more DH5αTs to make more competent cells

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02/13/2014

Golden gate colonies

  • 1-4 did not grow
    • Realized the Lux boxes were not correct. Ordered new ones
  • 5-6 grew >10 small colonies
    • Picked two from each plate into 3mL LB+amp
  • Positive control grew hundreds of medium sized colonies



Got some more K12s from Kylie to make competent cells. Picked more DH5αTs to make more competent cells



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