Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/02/14

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Current revision (16:16, 14 February 2014) (view source)
(02/14/2014)
 
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==02/14/2014==
==02/14/2014==
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Miniprep cultures picked yesterday. Golden gate seems to have been successful because the liquid cultures were clearly red. This new part is pTet-B0034-mCh-T-T in pSB1A3. This will be used to build new senders.  
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Miniprep cultures picked yesterday. Golden gate seems to have been successful because the liquid cultures were clearly red. This new part is pTet-B0034-mCh-T-T in pSB1A3. This will be used to build new senders. <br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
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| align="center" style="background:#f0f0f0;"|'''260'''
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| align="center" style="background:#f0f0f0;"|'''280'''
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| align="center" style="background:#f0f0f0;"|'''260/280'''
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| align="center" style="background:#f0f0f0;"|'''ng/µL'''
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|-
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| pTet-B0034-mCh-T-T in pSB1A3 5-1||0.108||0.056||1.927||108.225
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|-
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| pTet-B0034-mCh-T-T in pSB1A3 6-2||0.077||0.039||1.986||76.508
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|-
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| pTet-B0034-mCh-T-T in pSB1A3 6-1||0.074||0.036||2.058||74.252
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|-
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| pTet-B0034-mCh-T-T in pSB1A3 5-2||0.102||0.05||2.021||101.873
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|-
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|
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|}
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Prepare K12 and DH5αT competent cells.
Prepare K12 and DH5αT competent cells.

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02/14/2014

Miniprep cultures picked yesterday. Golden gate seems to have been successful because the liquid cultures were clearly red. This new part is pTet-B0034-mCh-T-T in pSB1A3. This will be used to build new senders.

Sample Read# 260 280 260/280 ng/µL
pTet-B0034-mCh-T-T in pSB1A3 5-10.1080.0561.927108.225
pTet-B0034-mCh-T-T in pSB1A3 6-20.0770.0391.98676.508
pTet-B0034-mCh-T-T in pSB1A3 6-10.0740.0362.05874.252
pTet-B0034-mCh-T-T in pSB1A3 5-20.1020.052.021101.873



Prepare K12 and DH5αT competent cells.



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