Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/01: Difference between revisions

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{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Thing'''
| align="center" style="background:#f0f0f0;"|'''Volume (ul)'''
|-
| Part I||1
|-
| Part II||1
|-
| Part III||1
|-
| T5 ligase||1.25
|-
| BsmBI||0.5
|-
| T4 DNA ligase buffer||1
|-
| H2O||4.25
|}
<br>
GG reaction
<br>
Transformed into new DH5alphaT made by me soon after reaction finished. Long transformation, heat shock, 1.5 hour recovery time. New plates made by Behzad. Positive control plasmid, negative control of water. 
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__NOTOC__
__NOTOC__

Revision as of 13:50, 7 April 2014

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04/01/2014

Sample Read# ng/µL fmol/ul ul Sample ul H2O
I13522 P111 P12 38.172 25.15384615 7.951070336 2.048929664
G001 P105 P107 5.052 76.53846154 2.613065327 7.386934673
Thing Volume (ul)
Part I 1
Part II 1
Part III 1
T5 ligase 1.25
BsmBI 0.5
T4 DNA ligase buffer 1
H2O 4.25


GG reaction
Transformed into new DH5alphaT made by me soon after reaction finished. Long transformation, heat shock, 1.5 hour recovery time. New plates made by Behzad. Positive control plasmid, negative control of water.


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