Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/04/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
(fix raw html notebook nav)
 
(6 intermediate revisions by one other user not shown)
Line 2: Line 2:
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==mm/dd/yyyy==
==mm/dd/yyyy==
* Insert content here...
PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Number'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
| align="center" style="background:#f0f0f0;"|'''Tm'''
| align="center" style="background:#f0f0f0;"|'''Expected length'''
| align="center" style="background:#f0f0f0;"|'''Expected incorrect length'''
| align="center" style="background:#f0f0f0;"|'''Product'''
|-
| 1||Receiver int||P123||P112||49||2203||2430||Rec backbone
|-
|2|| Receiver int||P119||P107||48||1051||||pLux
|-
| 3||Receiver int||P125||P107||48||1051||||pRhl
|-
| 4||Receiver int||P126||P107||48||1051||||pEsa
|-
|5|| F2620||P113||P114||51||817||||LuxR
|-
| 6||K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
|-
| 7||K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
|-
|8|| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
|-
| 9||EsaR synthesized||P121||P122||50||758||||EsaR
|}


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Latest revision as of 23:56, 26 September 2017

Project name Main project page
Previous entry      Next entry

mm/dd/yyyy

PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Number Template F primer R primer Tm Expected length Expected incorrect length Product
1 Receiver int P123 P112 49 2203 2430 Rec backbone
2 Receiver int P119 P107 48 1051 pLux
3 Receiver int P125 P107 48 1051 pRhl
4 Receiver int P126 P107 48 1051 pEsa
5 F2620 P113 P114 51 817 LuxR
6 K092900+eCFP (70ul/ng) P117 P118 67 765 RhlR
7 K092900+eCFP (74ng/ul) P117 P118 67 765 RhlR
8 K092900 (96ng/ul) P117 P118 67 765 RhlR
9 EsaR synthesized P121 P122 50 758 EsaR


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs/2014/04/28&oldid=1014496"