Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28

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(Autocreate 2014/04/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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PCR for Receiver assemblies
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Template'''
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| align="center" style="background:#f0f0f0;"|'''F primer'''
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| align="center" style="background:#f0f0f0;"|'''R primer'''
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| align="center" style="background:#f0f0f0;"|'''Expected length'''
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| align="center" style="background:#f0f0f0;"|'''Expected incorrect length'''
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| align="center" style="background:#f0f0f0;"|'''Product'''
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|-
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| Receiver int||P123||P112||2203||2430||Rec backbone
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|-
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| Receiver int||P119||P107||1051||||pLux
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|-
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| Receiver int||P125||P108||1051||||pRhl
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|-
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| Receiver int||P126||P109||1051||||pEsa
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|-
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| F2620||P113||P114||817||||LuxR
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|-
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| K092900+eCFP||P117||P118||765||||RhlR
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|-
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| EsaR synthesized||P121||P122||758||||EsaR
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|}
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Revision as of 19:04, 28 April 2014

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mm/dd/yyyy

PCR for Receiver assemblies

Template F primer R primer Expected length Expected incorrect length Product
Receiver intP123P11222032430Rec backbone
Receiver intP119P1071051pLux
Receiver intP125P1081051pRhl
Receiver intP126P1091051pEsa
F2620P113P114817LuxR
K092900+eCFPP117P118765RhlR
EsaR synthesizedP121P122758EsaR


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