Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28

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| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
 +
| align="center" style="background:#f0f0f0;"|'''Tm'''
| align="center" style="background:#f0f0f0;"|'''Expected length'''
| align="center" style="background:#f0f0f0;"|'''Expected length'''
| align="center" style="background:#f0f0f0;"|'''Expected incorrect length'''
| align="center" style="background:#f0f0f0;"|'''Expected incorrect length'''
| align="center" style="background:#f0f0f0;"|'''Product'''
| align="center" style="background:#f0f0f0;"|'''Product'''
|-
|-
-
| Receiver int||P123||P112||2203||2430||Rec backbone
+
| Receiver int||P123||P112||49||2203||2430||Rec backbone
|-
|-
-
| Receiver int||P119||P107||1051||||pLux
+
| Receiver int||P119||P107||48||1051||||pLux
|-
|-
-
| Receiver int||P125||P107||1051||||pRhl
+
| Receiver int||P125||P107||48||1051||||pRhl
|-
|-
-
| Receiver int||P126||P107||1051||||pEsa
+
| Receiver int||P126||P107||48||1051||||pEsa
|-
|-
-
| F2620||P113||P114||817||||LuxR
+
| F2620||P113||P114||51||817||||LuxR
|-
|-
-
| K092900+eCFP||P117||P118||765||||RhlR
+
| K092900+eCFP||P117||P118||67||765||||RhlR
|-
|-
-
| EsaR synthesized||P121||P122||758||||EsaR
+
| EsaR synthesized||P121||P122||50||758||||EsaR
|}
|}

Revision as of 18:51, 28 April 2014

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PCR for Receiver assemblies

Template F primer R primer Tm Expected length Expected incorrect length Product
Receiver intP123P1124922032430Rec backbone
Receiver intP119P107481051pLux
Receiver intP125P107481051pRhl
Receiver intP126P107481051pEsa
F2620P113P11451817LuxR
K092900+eCFPP117P11867765RhlR
EsaR synthesizedP121P12250758EsaR


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