Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28

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(Autocreate 2014/04/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (14:49, 29 April 2014) (view source)
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PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Number'''
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| align="center" style="background:#f0f0f0;"|'''Template'''
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| align="center" style="background:#f0f0f0;"|'''F primer'''
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| align="center" style="background:#f0f0f0;"|'''R primer'''
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| align="center" style="background:#f0f0f0;"|'''Tm'''
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| align="center" style="background:#f0f0f0;"|'''Expected length'''
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| align="center" style="background:#f0f0f0;"|'''Expected incorrect length'''
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| align="center" style="background:#f0f0f0;"|'''Product'''
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|-
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| 1||Receiver int||P123||P112||49||2203||2430||Rec backbone
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|-
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|2|| Receiver int||P119||P107||48||1051||||pLux
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|-
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| 3||Receiver int||P125||P107||48||1051||||pRhl
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|-
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| 4||Receiver int||P126||P107||48||1051||||pEsa
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|-
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|5|| F2620||P113||P114||51||817||||LuxR
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|-
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| 6||K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
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|-
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| 7||K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
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|-
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|8|| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
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|-
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| 9||EsaR synthesized||P121||P122||50||758||||EsaR
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|}
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mm/dd/yyyy

PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Number Template F primer R primer Tm Expected length Expected incorrect length Product
1Receiver intP123P1124922032430Rec backbone
2 Receiver intP119P107481051pLux
3Receiver intP125P107481051pRhl
4Receiver intP126P107481051pEsa
5 F2620P113P11451817LuxR
6K092900+eCFP (70ul/ng)P117P11867765RhlR
7K092900+eCFP (74ng/ul)P117P11867765RhlR
8 K092900 (96ng/ul)P117P11867765RhlR
9EsaR synthesizedP121P12250758EsaR


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