Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28

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| F2620||P113||P114||51||817||||LuxR
| F2620||P113||P114||51||817||||LuxR
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|-
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| K092900+eCFP||P117||P118||67||765||||RhlR
+
| K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
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|-
 +
| K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
 +
|-
 +
| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
|-
|-
| EsaR synthesized||P121||P122||50||758||||EsaR
| EsaR synthesized||P121||P122||50||758||||EsaR

Revision as of 15:01, 29 April 2014

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PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Template F primer R primer Tm Expected length Expected incorrect length Product
Receiver intP123P1124922032430Rec backbone
Receiver intP119P107481051pLux
Receiver intP125P107481051pRhl
Receiver intP126P107481051pEsa
F2620P113P11451817LuxR
K092900+eCFP (70ul/ng)P117P11867765RhlR
K092900+eCFP (74ng/ul)P117P11867765RhlR
K092900 (96ng/ul)P117P11867765RhlR
EsaR synthesizedP121P12250758EsaR


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