Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/28

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(mm/dd/yyyy)
Current revision (14:49, 29 April 2014) (view source)
(mm/dd/yyyy)
 
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{| {{table}}
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Number'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
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| align="center" style="background:#f0f0f0;"|'''Product'''
| align="center" style="background:#f0f0f0;"|'''Product'''
|-
|-
-
| Receiver int||P123||P112||49||2203||2430||Rec backbone
+
| 1||Receiver int||P123||P112||49||2203||2430||Rec backbone
|-
|-
-
| Receiver int||P119||P107||48||1051||||pLux
+
|2|| Receiver int||P119||P107||48||1051||||pLux
|-
|-
-
| Receiver int||P125||P107||48||1051||||pRhl
+
| 3||Receiver int||P125||P107||48||1051||||pRhl
|-
|-
-
| Receiver int||P126||P107||48||1051||||pEsa
+
| 4||Receiver int||P126||P107||48||1051||||pEsa
|-
|-
-
| F2620||P113||P114||51||817||||LuxR
+
|5|| F2620||P113||P114||51||817||||LuxR
|-
|-
-
| K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
+
| 6||K092900+eCFP (70ul/ng)||P117||P118||67||765||||RhlR
|-
|-
-
| K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
+
| 7||K092900+eCFP (74ng/ul)||P117||P118||67||765||||RhlR
|-
|-
-
| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
+
|8|| K092900 (96ng/ul)||P117||P118||67||765||||RhlR
|-
|-
-
| EsaR synthesized||P121||P122||50||758||||EsaR
+
| 9||EsaR synthesized||P121||P122||50||758||||EsaR
|}
|}

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mm/dd/yyyy

PCR for Receiver assemblies. Diluted all templates 1:1000 in water, annealing temp 48, extension time 2:20

Number Template F primer R primer Tm Expected length Expected incorrect length Product
1Receiver intP123P1124922032430Rec backbone
2 Receiver intP119P107481051pLux
3Receiver intP125P107481051pRhl
4Receiver intP126P107481051pEsa
5 F2620P113P11451817LuxR
6K092900+eCFP (70ul/ng)P117P11867765RhlR
7K092900+eCFP (74ng/ul)P117P11867765RhlR
8 K092900 (96ng/ul)P117P11867765RhlR
9EsaR synthesizedP121P12250758EsaR


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