Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/29: Difference between revisions
Rene M Davis (talk | contribs) |
(fix raw html notebook nav) |
||
(2 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
Line 48: | Line 48: | ||
<br> | <br> | ||
Gel extracted PCR reaction 1. Saw a smaller band above, think I got the correct band. | Gel extracted PCR reaction 1. Saw a smaller band above, think I got the correct band. | ||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''PCR reaction''' | |||
| align="center" style="background:#f0f0f0;"|'''Description''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| 1||Rec bb||Gel extracted||0.02||0.01||2||20.289 | |||
|- | |||
| 2||pLux-GFP||DpnI digested, column purified||0.047||0.024||2||47.148 | |||
|- | |||
| 3||pRhl-GFP||DpnI digested, column purified||0.05||0.025||2.004||50.466 | |||
|- | |||
| 4||pEsa-GFP||DpnI digested, column purified||0.032||0.015||2.117||32.1 | |||
|- | |||
| 5||pLac-LuxR||DpnI digested, column purified||0.046||0.024||1.935||45.771 | |||
|- | |||
| 6||pLac-RhlR||DpnI digested, column purified||0.06||0.035||1.692||59.88 | |||
|- | |||
| 7||pLac-RhlR||DpnI digested, column purified||0.041||0.019||2.2||41.438 | |||
|- | |||
| 9||pLac-EsaR||DpnI digested, column purified||0.038||0.018||2.135||37.847 | |||
|- | |||
| | |||
|} | |||
Didn't have enough of the backbone so started another PCR. 1-4 are int receiver vector P112 P123, 5-6 are purified PCR product of receiver backbone P112 P123. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | ||
|} | |} | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 23:56, 26 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
mm/dd/yyyyRan PCR products from yesterday on a gel. 5ul of reactions 2-9, all 30 of 1 so I can gel extract because there should be two bands and I only want one.
Incubated at 37°C for 55 minutes, sat on the bench for 3 minutes, heat inactivated at 80 for 5 minutes.
Didn't have enough of the backbone so started another PCR. 1-4 are int receiver vector P112 P123, 5-6 are purified PCR product of receiver backbone P112 P123. |