Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/29: Difference between revisions
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Gel extracted PCR reaction 1. Saw a smaller band above, think I got the correct band. | Gel extracted PCR reaction 1. Saw a smaller band above, think I got the correct band. | ||
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''PCR reaction''' | |||
| align="center" style="background:#f0f0f0;"|'''Description''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
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| 1||Rec bb||Gel extracted||0.02||0.01||2||20.289 | |||
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| 2||pLux-GFP||DpnI digested, column purified||0.047||0.024||2||47.148 | |||
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| 3||pRhl-GFP||DpnI digested, column purified||0.05||0.025||2.004||50.466 | |||
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| 4||pEsa-GFP||DpnI digested, column purified||0.032||0.015||2.117||32.1 | |||
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| 5||pLac-LuxR||DpnI digested, column purified||0.046||0.024||1.935||45.771 | |||
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| 6||pLac-RhlR||DpnI digested, column purified||0.06||0.035||1.692||59.88 | |||
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| 7||pLac-RhlR||DpnI digested, column purified||0.041||0.019||2.2||41.438 | |||
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| 9||pLac-EsaR||DpnI digested, column purified||0.038||0.018||2.135||37.847 | |||
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Revision as of 13:15, 29 April 2014
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mm/dd/yyyyRan PCR products from yesterday on a gel. 5ul of reactions 2-9, all 30 of 1 so I can gel extract because there should be two bands and I only want one.
Incubated at 37°C for 55 minutes, sat on the bench for 3 minutes, heat inactivated at 80 for 5 minutes.
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