Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/04/30: Difference between revisions
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Couldn't see the PCR products on the gel, maybe I ran it too long? 1 hour at 100, 1.5 more at 85. Half gel. Should have at least been able to see the the top of the 10kb ladder but I couldn't see anything. Wondering if the gel box UV light is bad. | |||
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Transformation of golden gate reactions. New cells made with Behzad. Used 70ul for each of 5 transformations. 30 minutes on ice, 30s at 42, 2 min ice, 1 hour shaking SOC. | |||
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Revision as of 15:45, 30 April 2014
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mm/dd/yyyyPurified PCR reactions 5 and 6 from yesterday (receiver backbone) using the gel extraction columns and the DNA clean and concentrator kit. Got really low yield, need to look into a better kit for this.
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