Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/06: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2014/05/06 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==mm/dd/yyyy== | ==mm/dd/yyyy== | ||
Ryan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000. | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Number''' | |||
| align="center" style="background:#f0f0f0;"|'''Template''' | |||
| align="center" style="background:#f0f0f0;"|'''F Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R Primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Annealing T''' | |||
| align="center" style="background:#f0f0f0;"|'''Expected length''' | |||
|- | |||
| 1||pTet-mCh 2/14/14 74ng||P127||P097||53||3148 | |||
|- | |||
| 2||LuxI sender const 8/31/13 179ng||P063||P064||23||654 | |||
|- | |||
| 3||K084007 3/10/13 4-2-1||P069||P070||32||645 | |||
|- | |||
| 4||K084007 3/10/13 4-2-2||P069||P070||32||645 | |||
|- | |||
| 5||RBS-LasI 3/8/13 31ng||P069||P070||32||645 | |||
|- | |||
| 6||RhlI sender const 9/27/13 72 ng||P075||P076||39||642 | |||
|} | |||
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<br> | |||
Ryan ran gel of colony PCRs from last week. | |||
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Latest revision as of 23:56, 26 September 2017
Project name | Main project page Previous entry Next entry | ||||||||||||||||||||||||||||||||||||||||||
mm/dd/yyyyRyan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000.
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