Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/06

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Current revision (12:04, 6 May 2014) (view source)
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Ryan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000.
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{| {{table}}
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Number'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''Template'''
| align="center" style="background:#f0f0f0;"|'''F Primer'''
| align="center" style="background:#f0f0f0;"|'''F Primer'''
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| align="center" style="background:#f0f0f0;"|'''Expected length'''
| align="center" style="background:#f0f0f0;"|'''Expected length'''
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|-
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| mCherry sender intermediate vector||P127||P097||53||3148
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| 1||pTet-mCh 2/14/14 74ng||P127||P097||53||3148
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|-
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| LuxI||P063||P064||23||
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| 2||LuxI sender const 8/31/13 179ng||P063||P064||23||654
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|-
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| LasI||P069||P070||32||
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| 3||K084007 3/10/13 4-2-1||P069||P070||32||645
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|-
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| RhlI||P075||P076||39||
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| 4||K084007 3/10/13 4-2-2||P069||P070||32||645
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|-
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| 5||RBS-LasI 3/8/13 31ng||P069||P070||32||645
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|-
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| 6||RhlI sender const 9/27/13 72 ng||P075||P076||39||642
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|}
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Ryan ran gel of colony PCRs from last week.
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mm/dd/yyyy

Ryan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000.

Number Template F Primer R Primer Annealing T Expected length
1pTet-mCh 2/14/14 74ngP127P097533148
2LuxI sender const 8/31/13 179ngP063P06423654
3K084007 3/10/13 4-2-1P069P07032645
4K084007 3/10/13 4-2-2P069P07032645
5RBS-LasI 3/8/13 31ngP069P07032645
6RhlI sender const 9/27/13 72 ngP075P07639642



Ryan ran gel of colony PCRs from last week.


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