Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/06

From OpenWetWare

< Haynes Lab:Notebook | Characterizing AHL quorum sensing homologs | 2014 | 05
Revision as of 13:04, 6 May 2014 by Rene M Davis (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

mm/dd/yyyy

Ryan set up PCR. Annealing temp 53, extension time 3:30min. Diluted each template down 1:1000.

Number Template F Primer R Primer Annealing T Expected length
1pTet-mCh 2/14/14 74ngP127P097533148
2LuxI sender const 8/31/13 179ngP063P06423654
3K084007 3/10/13 4-2-1P069P07032645
4K084007 3/10/13 4-2-2P069P07032645
5RBS-LasI 3/8/13 31ngP069P07032645
6RhlI sender const 9/27/13 72 ngP075P07639642



Ryan ran gel of colony PCRs from last week.


Personal tools