Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15

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| Receiver int vec||56.995||27||7.407407407
| Receiver int vec||56.995||27||7.407407407
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|}
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Reaction'''
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| align="center" style="background:#f0f0f0;"|'''Rec bb'''
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| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
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| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
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| align="center" style="background:#f0f0f0;"|'''BsmBI'''
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| align="center" style="background:#f0f0f0;"|'''T4 ligase'''
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| align="center" style="background:#f0f0f0;"|'''T4 ligase buffer'''
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| align="center" style="background:#f0f0f0;"|'''H2O'''
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|-
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| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||1.54
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|-
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| Rhl Rec||7.4||2.78||1.68||0.5||1.25||2||2.27
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|-
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| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||-0.32
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|-
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| Bb control||7.4||0||0||0.5||1.25||2||6.73
|}
|}
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Revision as of 13:03, 15 May 2014

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Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec56.995277.407407407


Reaction Rec bb pLux-GFP pLac-LuxR BsmBI T4 ligase T4 ligase buffer H2O
Lux Rec7.42.942.250.51.2521.54
Rhl Rec7.42.781.680.51.2522.27
Esa Rec7.44.352.70.51.252-0.32
Bb control7.4000.51.2526.73


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