Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15

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{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Reaction'''
| align="center" style="background:#f0f0f0;"|'''Reaction'''
-
| align="center" style="background:#f0f0f0;"|'''Rec bb'''
+
| align="center" style="background:#f0f0f0;"|'''Rec Bb'''
| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
| align="center" style="background:#f0f0f0;"|'''BsmBI'''
| align="center" style="background:#f0f0f0;"|'''BsmBI'''
-
| align="center" style="background:#f0f0f0;"|'''T4 ligase'''
+
| align="center" style="background:#f0f0f0;"|'''T4 Ligase'''
-
| align="center" style="background:#f0f0f0;"|'''T4 ligase buffer'''
+
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
|-
|-
-
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||1.54
+
| Lux Rec||7.4||2.94||2.25||0.5||1.25||2||3.66
|-
|-
-
| Rhl Rec||7.4||2.78||1.68||0.5||1.25||2||2.27
+
| Rhl Rec||7.4||2.78||1.68||0.5||1.25||2||4.39
|-
|-
-
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||-0.32
+
| Esa Rec||7.4||4.35||2.7||0.5||1.25||2||1.8
|-
|-
-
| Bb control||7.4||0||0||0.5||1.25||2||6.73
+
| Bb ctrl||7.4||0||0||0.5||1.25||2||8.85
|}
|}
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Revision as of 12:09, 15 May 2014

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mm/dd/yyyy

Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec56.995277.407407407


Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Lux Rec7.42.942.250.51.2523.66
Rhl Rec7.42.781.680.51.2524.39
Esa Rec7.44.352.70.51.2521.8
Bb ctrl7.4000.51.2528.85


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