Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/15

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Receiver golden gate with newly prepped receiver intermediate vector

Part ng/µL fmol/ul ml for 200fmol
Receiver int vec56.995277.407407407


Reaction Rec bb pLux-GFP pLac-LuxR BsmBI T4 ligase T4 ligase buffer H2O
Lux Rec7.42.942.250.51.2521.54
Rhl Rec7.42.781.680.51.2522.27
Esa Rec7.44.352.70.51.252-0.32
Bb control7.4000.51.2526.73


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