Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/19: Difference between revisions

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(Autocreate 2014/05/19 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==mm/dd/yyyy==
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10:1 control plate has 0 colonies, 10:1 LuxR receiver plate has maybe 7 or so colonies (some are very small, and several of the plates have many colony-sized bubbles making it hard to count). Picked four colonies off X 10:1 plate to miniprep tomorrow.  
 
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Golden gate ligations for other receivers with 10:1 insert to bb ratio
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Reaction'''
| align="center" style="background:#f0f0f0;"|'''Rec Bb'''
| align="center" style="background:#f0f0f0;"|'''pLux-GFP'''
| align="center" style="background:#f0f0f0;"|'''pLac-LuxR'''
| align="center" style="background:#f0f0f0;"|'''BsmBI'''
| align="center" style="background:#f0f0f0;"|'''T4 Ligase'''
| align="center" style="background:#f0f0f0;"|'''T4 Ligase buffer'''
| align="center" style="background:#f0f0f0;"|'''H2O'''
|-
| Bb ctrl||0.74||0||0||0.5||1.25||2||5.51
|-
| Rhl Rec||0.74||2.78||1.68||0.5||1.25||2||1.05
|-
| Esa Rec||0.74||4.35||2.7||0.5||1.25||2||-1.54
|}


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Revision as of 15:03, 19 May 2014

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mm/dd/yyyy

10:1 control plate has 0 colonies, 10:1 LuxR receiver plate has maybe 7 or so colonies (some are very small, and several of the plates have many colony-sized bubbles making it hard to count). Picked four colonies off X 10:1 plate to miniprep tomorrow.

Golden gate ligations for other receivers with 10:1 insert to bb ratio

Reaction Rec Bb pLux-GFP pLac-LuxR BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl 0.74 0 0 0.5 1.25 2 5.51
Rhl Rec 0.74 2.78 1.68 0.5 1.25 2 1.05
Esa Rec 0.74 4.35 2.7 0.5 1.25 2 -1.54


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