Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/21: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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Ran above PCR on gel | Ran above PCR on gel | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Template''' | |||
| align="center" style="background:#f0f0f0;"|'''F primer''' | |||
| align="center" style="background:#f0f0f0;"|'''R primer''' | |||
| align="center" style="background:#f0f0f0;"|'''Expected band size''' | |||
| align="center" style="background:#f0f0f0;"|'''Actual band size''' | |||
|- | |||
| pTet-mCh||130||97||3130||3130 | |||
|- | |||
| LuxI GFP rec||131||132||610||no band | |||
|- | |||
| LuxR 1:10 colony 1||103||114||1817||no band | |||
|- | |||
| LuxR 1:10 colony 2||103||114||1817||1817 | |||
|- | |||
| LuxR 1:10 colony 3||103||114||1817||no band | |||
|- | |||
| LuxR 1:10 colony 4||103||114||1817||no band | |||
|- | |||
| LuxR 1:10 colony 5||103||114||1817||1817 | |||
|- | |||
| LuxR 1:10 colony 5||103||107||1035||1035 | |||
|} | |||
<br><br> | <br><br> | ||
picked LuxI and RhlI GFP senders into 4mLs media and shook overnight to screen for successful receiver transformations. | picked LuxI and RhlI GFP senders into 4mLs media and shook overnight to screen for successful receiver transformations. | ||
Revision as of 16:56, 22 May 2014
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
mm/dd/yyyyGolden gate 1:10 RhlR and EsaR receivers grew but there were colonies on the backbone plate
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