Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/22

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yyyy

PCR clean up of EsaI PCR and mCh Sender Intermediate for golden gate. Forgot to do DpnI digest of mCh (don't need to for EsaI because the PCR template is kan resistance).

Sample 260 280 260/280 ng/µL
mCh Sender int gg 0.055 0.031 1.774 55.337
EsaI 0.046 0.026 1.776 45.692



Going to run golden gate anyway. Backbone plate should have the same number of PCR template transformants as any of the ligation plates.
200fmol of insert, 20fmol backbone

Reaction Rec Bb LuxI BsmBI T4 Ligase T4 Ligase buffer H2O
Bb ctrl 0.77 0 0.5 1.25 2 15.48
RhlI 0.77 3.2 0.5 1.25 2 12.28
LasI 0.77 2.8 0.5 1.25 2 12.68
EsaI 0.77 1.9 0.5 1.25 2 13.58


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs/2014/05/22&oldid=794575"