Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/05/23: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) (Autocreate 2014/05/23 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
Rene M Davis (talk | contribs) |
||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==mm/dd/yyyy== | ==mm/dd/yyyy== | ||
Picked each of the colonies on the receiver plates I made trying to test if any of the constructs were correct. I picked a big swab of each colony and swirled it in some PBS. Then I ran each of them on the flow cytometer. I used the senders (which definitely have GFP) as positive controls. The RhlI sender was green and every. other. colony. was not. This is clearly not a clever and efficient way to screen for receiver constructs. | |||
<br><br> | |||
Possible reasons for failure:<br> | |||
:All of the receiver constructs are incorrect. | |||
:Receivers are making very low amounts of GFP because of their medium RBS, reduction of read-through expression, and being in DH5alphas. | |||
:Senders were not making much AHL because they're in DH5alphas. | |||
:Maybe I didn't leave them long enough in the incubator. | |||
Revision as of 15:44, 29 May 2014
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
mm/dd/yyyyPicked each of the colonies on the receiver plates I made trying to test if any of the constructs were correct. I picked a big swab of each colony and swirled it in some PBS. Then I ran each of them on the flow cytometer. I used the senders (which definitely have GFP) as positive controls. The RhlI sender was green and every. other. colony. was not. This is clearly not a clever and efficient way to screen for receiver constructs.
| |
