Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/07/06

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Current revision (16:13, 8 July 2014) (view source)
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Results from yesterday's ligation <br>
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''Backbone'''
 
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| align="center" style="background:#f0f0f0;"|'''Insert'''
 
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| align="center" style="background:#f0f0f0;"|'''# colonies'''
 
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| pX330||bb ctrl||13
 
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| pX330||g14||30
 
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|-
 
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| pX330||g5||20
 
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|-
 
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| pX330||g13||29
 
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|-
 
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| pX330||gn1T||21
 
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|-
 
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| pX330||gn1B||8
 
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|-
 
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| pX330||gn3T||22
 
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|-
 
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| pX330||gnBb B||20
 
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|-
 
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| pX335||bb ctrl||19
 
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| pX335||gn3T||14
 
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|-
 
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| pX335||gn1B||19
 
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| pX335||gn1T||23
 
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| pX335||gn3bB||23
 
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|}
 
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<br><br>
 
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Zhang lab help pages say overnight ligations can lead to many colonies on the backbone plate. Picked 6 colonies from plate pX330-g14 plate.
 
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