Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2014/10/08: Difference between revisions

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==mm/dd/yyyy==
==mm/dd/yyyy==
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* Retried PCR of golden gate parts using Rene's primers, this time adding DMSO
* Retried PCR of golden gate parts using Rene's primers, this time adding DMSO


Revision as of 13:23, 12 October 2015

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mm/dd/yyyy

  • Retried PCR of golden gate parts using Rene's primers, this time adding DMSO
Reagent uL
ddH2O 31.5
HF buffer 10
primer F (10uM) 2.5
primer R (10uM) 2.5
dNTP (10mM) 1
Template K084007 0.5
DMSO 1.5
Phusion polymerase 0.5
Total 50



  • annealed for 30 seconds
Number Template F Primer R Primer Annealing T Expected length
1 K084007 3/10/13 4-2-1 P069 P070 32 645
2 K084007 3/10/13 4-2-2 P069 P070 32 645



  • Still no clear bands observed.



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