Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/02/23: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==02//2015==
==02/23/2015==
<font color = purple>
Today I prepared the liquid colonies that I made on Friday for sequencing. I transferred the liquid to 2mL tubes and spun the tubes, poured out the LB broth and isolated the pellet of cells. Then I mini prepped them. The following shows the concentrations of each tube after the mini-prep. <br>
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
|-
| 6||0.06||0.033||1.832||60.226
|-
| 8||0.06||0.033||1.789||59.697
|-
| 9||0.058||0.032||1.792||57.778
|-
| 10||0.059||0.033||1.785||59.318
|-
|}
<br>
Rene sent the samples out for sequencing later in the day. </font>
 




Latest revision as of 00:47, 27 September 2017

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02/23/2015

Today I prepared the liquid colonies that I made on Friday for sequencing. I transferred the liquid to 2mL tubes and spun the tubes, poured out the LB broth and isolated the pellet of cells. Then I mini prepped them. The following shows the concentrations of each tube after the mini-prep.

Sample Read# 260 280 260/280 ng/µL
6 0.06 0.033 1.832 60.226
8 0.06 0.033 1.789 59.697
9 0.058 0.032 1.792 57.778
10 0.059 0.033 1.785 59.318


Rene sent the samples out for sequencing later in the day.




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