Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/19: Difference between revisions

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==03/19/2015==
==03/19/2015==
<font color = purple>


<br>
Today I mini-prepped the ligation from 3/17/2015<br>
Mini prep of the ligation performed 3/17/2015<br>
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| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
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| lig 3:1||0.113||0.061||1.868||113.215
| lig 3:1||0.113||0.061||1.868||113.215
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After the mini-prep, I digested the plasmid with PstI and EcoR1. Then I conducted gel electrophoresis to see results of the digestion. <br>
Unfortunately, I accidentally inputted loading dye instead of a ladder, so I will have to redo the digestion and gel tomorrow. <br>
 




Latest revision as of 00:50, 27 September 2017

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03/19/2015

Today I mini-prepped the ligation from 3/17/2015

Sample Read# 260 280 260/280 ng/µL
lig 3:1 0.113 0.061 1.868 113.215


After the mini-prep, I digested the plasmid with PstI and EcoR1. Then I conducted gel electrophoresis to see results of the digestion.
Unfortunately, I accidentally inputted loading dye instead of a ladder, so I will have to redo the digestion and gel tomorrow.




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