Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/03/31: Difference between revisions
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more | Today Ryan double digested more backbone with EcoRI and XbaI for the second ligation. <br> | ||
eluted at 45uL with warm water | I PCR purified the reactions and eluted at 45uL with warm water. <br> <br> | ||
After purification, I pipetted the following contents into PCR tubes: <br> | |||
*note to self: use 2.0mL tubes next time if you are going straight into a transformation. <br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''Concentration (ng/uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of 62.5ng Insert (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of 50ng backbone (uL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Water''' | |||
|- | |||
| AmbI||29||2.16||4.17||10.67 | |||
|- | |||
| BjaI||30||2.08||4.17||10.75 | |||
|- | |||
| BraI||66||0.95||4.17||11.88 | |||
|- | |||
| CerI||50||1.25||4.17||11.58 | |||
|- | |||
| RpaI||30||2.08||4.17||10.75 | |||
|- | |||
| EsaI||39||1.6||4.17||11.23 | |||
|- | |||
| LuxI||45||1.39||4.17||11.44 | |||
|- | |||
| LasI||36||1.74||4.17||11.09 | |||
|- | |||
| SinI||47||1.33||4.17||11.5 | |||
|- | |||
| Bb only ||39||1.6||4.17||11.23 | |||
|} | |||
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Revision as of 19:00, 31 March 2015
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03/31/2015
Today Ryan double digested more backbone with EcoRI and XbaI for the second ligation. After purification, I pipetted the following contents into PCR tubes:
|