Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/03: Difference between revisions
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Ligate modular receiver vector (MRV) + RhlR, LuxR, and EsaR. <br> | <h2> Ligate modular receiver vector (MRV) + RhlR, LuxR, and EsaR. </h2> <br> | ||
<b>1. Digest 1ug MRV </b> | <b>1. Digest 1ug MRV </b> | ||
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Revision as of 17:48, 3 July 2015
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06/3/2015
Ligate modular receiver vector (MRV) + RhlR, LuxR, and EsaR.1. Digest 1ug MRV
Incubate at 37°C for 15 minutes. 2. Digest PCR amplified and purified, regulators w/ E+X.
3. Ligate 3:1 ratio of insert:bb
Amounts of DNA used were based on vector/insert size.
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