Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/24: Difference between revisions
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<b>Miniprep: LasR 1-6, AubR 1-4, EsaR 2, 8. (12)</b> | <b>Miniprep: LasR 1-6, AubR 1-4, EsaR 2, 8. (12)</b> | ||
<b>Set up colony PCR for pLux_MRV + LuxR </b> | |||
:8 completed plasmids and 3 backbones used as a control | |||
<b>Digest verify LasR 1-6, AubR 1-4, Esa 2, 8. </b> | <b>Digest verify LasR 1-6, AubR 1-4, Esa 2, 8. </b> | ||
:[[Image:15.07.24_digest_verifications.TIF.TIF|600px]] | :[[Image:15.07.24_digest_verifications.TIF.TIF|600px]] | ||
:Sent LasR 1, 3, and AubR 4 out for sequencing. It looks like AubR 4 is successful. | |||
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Revision as of 16:35, 27 July 2015
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07/24/2015Miniprep: LasR 1-6, AubR 1-4, EsaR 2, 8. (12) Set up colony PCR for pLux_MRV + LuxR
Digest verify LasR 1-6, AubR 1-4, Esa 2, 8.
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