Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2015/07/27: Difference between revisions

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<b> Gel-verify pLux_MRV + LuxR's colony PCR from Friday. </b>  
<b> Gel-verify pLux_MRV + LuxR's colony PCR from Friday. </b>  
 
:[[Image:15.07.27 pLux colony pcr gel and pLas at the end.TIF|600px]]
<b> Make more liquid cultures for: pEsa_MRV + EsaR, and RhlI. </b>
:For Lux, colonies 1, 2, 4, 6, 7, and 8 seem to be successful
 
<b> Make more liquid cultures for: pEsa_MRV + EsaR and RhlI. </b>
:*For Esa, colonies: 1, 2, 3, 5, 6, 7, 8 appear to be successful according to their colony PCR gel image.


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Latest revision as of 01:04, 27 September 2017

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07/27/2015

Gel-verify pLux_MRV + LuxR's colony PCR from Friday.

For Lux, colonies 1, 2, 4, 6, 7, and 8 seem to be successful

Make more liquid cultures for: pEsa_MRV + EsaR and RhlI.

  • For Esa, colonies: 1, 2, 3, 5, 6, 7, 8 appear to be successful according to their colony PCR gel image.



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