Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/06/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
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Latest revision as of 01:50, 27 September 2017
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06/27/2016
In lab meeting, René suggested PCR-purifying the pLas insert after digestion, rather than heat inactivating. Since the insert is a small sequence, the high heat may not have annealed properly after denaturing. I will go directly from PCR reaction to digestion with EcoRI and SpeI, and then PCR purify after a gel verification. I will digest LasR_MRV with BbsI, heat inactivate and then PCR purify. (Instead of doing a gel extraction).
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