Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2016/06/28: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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:Heat inactivate rSAP at 75°C for 2 minutes | :Heat inactivate rSAP at 75°C for 2 minutes | ||
:Heat inactivate BbsI at 65°C for 20 minutes | :Heat inactivate BbsI at 65°C for 20 minutes | ||
*Digest pCer and pBta3R | |||
:{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume (uL)''' | |||
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| DNA (500ng)||12 | |||
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| 10x Green FD Buffer||3 | |||
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| EcoRI || 1 | |||
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| SpeI || 1 | |||
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| Water||13 | |||
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| Total||30 | |||
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:Incubate at 37°C for 10-20 minutes | |||
:PCR purify | |||
*PCR pExp and pLas | *PCR pExp and pLas |
Latest revision as of 01:50, 27 September 2017
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06/28/2016
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