Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/09/11: Difference between revisions
(Autocreate 2013/09/11 Entry for Haynes_Lab:Notebook/David_Barclay_Undergrad_Training/Plasmid_Transformation) |
(No difference)
|
Revision as of 08:18, 11 September 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Tuesday, June 25thSteps for TransformationUsing KAH06 for Plasmid. Notes
Friday, June 28thMiniprepAims: Run plasmids through miniprep in order to isolate plasmid DNA and determine concentration. Protocol: A. Pre-Miniprep1. Add 3 mL LB into 15 mL tube B. Miniprep1. Pipette 1.5 mL of sample into 2 mL tube. Spin for 3 min @ max setting (13.1g). Repeat once more to spin entire sample. C. DNA Concentration- Take 3 Plate1. Open program Gen 5 2.0 before machine. Results
Causes of Errors
|