Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/03: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(2 intermediate revisions by the same user not shown) | |||
Line 23: | Line 23: | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. fshPCD = 186 bp, 4968 bp<br> | | rowspan="7" | <u>Expected:</u><br>1. fshPCD = 186 bp, 4968 bp<br> | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:Gel DKB 10-3-13.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA(plasmid) || 5.0 μL | | DNA(plasmid) || 5.0 μL | ||
Line 38: | Line 38: | ||
|} | |} | ||
'''Conclusion''' | '''Conclusion''' | ||
There were faint bands at the 186 position; however, they are harder to see on the picture. The 4968 bands for all 3 are very visible. Fairly successful digest. | |||
'''DNA Sequencing Samples''' | '''DNA Sequencing Samples''' | ||
<!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | <!-- * signs create an automatically bulleted list. Replace 'date' with the date you submitted the samples --> | ||
<!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | <!-- The Order Number is shown at the top of your sequencing order form. Record in the indicated spot below --> | ||
* Submitted to DNASU on: | * Submitted to DNASU on: 10/7/2013 | ||
* Order Number: | * Order Number: 8213 | ||
<!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | <!-- # signs create an automatically numbered list. Replace Plasmid 1 & 2 with the names of your plasmids. Replace 'name' with the appropriate name of each primer. --> | ||
# Plasmid 1 (fshPCD) - Forward P001 | # Plasmid 1 (fshPCD) - Forward P001 |
Revision as of 14:17, 7 October 2013
Haynes BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||
Continuation of Restriction Digests and Gel ElectrophoresisRestriction Digest Table
Conclusion There were faint bands at the 186 position; however, they are harder to see on the picture. The 4968 bands for all 3 are very visible. Fairly successful digest. DNA Sequencing Samples
|