Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27

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(Autocreate 2013/10/27 Entry for Haynes_Lab:Notebook/David_Barclay_Undergrad_Training/Plasmid_Transformation)
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=UPLassay=
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*This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.
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==Reaction List==
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| '''REACTION LIST'''
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{| width=300px
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|-
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| &nbsp; || '''Template cDNA''' || '''Gene Target'''
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|-
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| Rxn 1: || treated cells || PDX1
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|-
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| Rxn 2: || treated cells || MAFA
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|-
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| Rxn 3: || treated cells || GLP1R
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|-
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| Rxn 4: || treated cells || PCSK1
 +
|-
 +
| Rxn 5: || treated cells || GAPD (reference gene)
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|-
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| Rxn 6: || untreated cells || PDX1
 +
|-
 +
| Rxn 7: || untreated cells || MAFA
 +
|-
 +
| Rxn 8: || untreated cells || GLP1R
 +
|-
 +
| Rxn 9: || untreated cells || PCSK1
 +
|-
 +
| Rxn 10: || untreated cells || GAPD (reference gene)
 +
|-
 +
| Rxn 11: || no template || PDX1
 +
|-
 +
| Rxn 12: || no template || MAFA
 +
|-
 +
| Rxn 13: || no template || GLP1R
 +
|-
 +
| Rxn 14: || no template || PCSK1
 +
|-
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| Rxn 15: || no template || GAPD (reference gene)
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|}
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Revision as of 00:58, 28 October 2013

Haynes BioBrick Cloning Main project page
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UPLassay

  • This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.

Reaction List

REACTION LIST
  Template cDNA Gene Target
Rxn 1: treated cells PDX1
Rxn 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells GAPD (reference gene)
Rxn 6: untreated cells PDX1
Rxn 7: untreated cells MAFA
Rxn 8: untreated cells GLP1R
Rxn 9: untreated cells PCSK1
Rxn 10: untreated cells GAPD (reference gene)
Rxn 11: no template PDX1
Rxn 12: no template MAFA
Rxn 13: no template GLP1R
Rxn 14: no template PCSK1
Rxn 15: no template GAPD (reference gene)




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