Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27: Difference between revisions

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(Autocreate 2013/10/27 Entry for Haynes_Lab:Notebook/David_Barclay_Undergrad_Training/Plasmid_Transformation)
 
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=UPLassay=
*This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.
==Reaction List==
| '''REACTION LIST'''
{| width=300px
|-
| &nbsp; || '''Template cDNA''' || '''Gene Target'''
|-
| Rxn 1: || treated cells || PDX1
|-
| Rxn 2: || treated cells || MAFA
|-
| Rxn 3: || treated cells || GLP1R
|-
| Rxn 4: || treated cells || PCSK1
|-
| Rxn 5: || treated cells || GAPD (reference gene)
|-
| Rxn 6: || untreated cells || PDX1
|-
| Rxn 7: || untreated cells || MAFA
|-
| Rxn 8: || untreated cells || GLP1R
|-
| Rxn 9: || untreated cells || PCSK1
|-
| Rxn 10: || untreated cells || GAPD (reference gene)
|-
| Rxn 11: || no template || PDX1
|-
| Rxn 12: || no template || MAFA
|-
| Rxn 13: || no template || GLP1R
|-
| Rxn 14: || no template || PCSK1
|-
| Rxn 15: || no template || GAPD (reference gene)
|}




Revision as of 22:58, 27 October 2013

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UPLassay

  • This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.

Reaction List

 REACTION LIST
  Template cDNA Gene Target
Rxn 1: treated cells PDX1
Rxn 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells GAPD (reference gene)
Rxn 6: untreated cells PDX1
Rxn 7: untreated cells MAFA
Rxn 8: untreated cells GLP1R
Rxn 9: untreated cells PCSK1
Rxn 10: untreated cells GAPD (reference gene)
Rxn 11: no template PDX1
Rxn 12: no template MAFA
Rxn 13: no template GLP1R
Rxn 14: no template PCSK1
Rxn 15: no template GAPD (reference gene)




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