Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27: Difference between revisions

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*Using Variation 2
*Using Variation 2
Variation 2<br> [[Image:Haynes_UPL_fig2.png|300px|Figure 1]]
Variation 2<br> [[Image:Haynes_UPL_fig2.png|300px|Figure 1]]
*Using as Reference, discount gene names on left side




Revision as of 23:02, 27 October 2013

Haynes BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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UPLassay

  • This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.

Reaction List

REACTION LIST
  Template cDNA Gene Target
Rxn 1: treated cells PDX1
Rxn 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells GAPD (reference gene)
Rxn 6: untreated cells PDX1
Rxn 7: untreated cells MAFA
Rxn 8: untreated cells GLP1R
Rxn 9: untreated cells PCSK1
Rxn 10: untreated cells GAPD (reference gene)
Rxn 11: no template PDX1
Rxn 12: no template MAFA
Rxn 13: no template GLP1R
Rxn 14: no template PCSK1
Rxn 15: no template GAPD (reference gene)
  • Run 3 Each
  • 45 Reactions Run
  • Using Variation 2

Variation 2
Figure 1

  • Using as Reference, discount gene names on left side
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