Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2013/10/27

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(Reaction List)
(Reaction List)
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*Using as Reference, discount gene names on left side
*Using as Reference, discount gene names on left side
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==Primers==
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*Created from Roche Applied Science https://www.roche-applied-science.com/shop/CategoryDisplay?catalogId=10001&tab=&identifier=Universal+Probe+Library&langId=-1#tab-3
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| '''PRIMERS LISt'''
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{| width=300px
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|-
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|   || '''Roche Name''' || '''Left Primer''' || '''Right Primer'''
 +
|-
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| PDX1 || Homo sapiens pancreatic and duodenal homeobox 1 (PDX1), mRNA NM_000209.3 || aagctcacgcgtggaaag || gccgtgagatgtacttgttgaa
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|-
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| Primer 2: || treated cells || MAFA
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|-
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| Rxn 3: || treated cells || GLP1R
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|-
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| Rxn 4: || treated cells || PCSK1
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|-
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| Rxn 5: || treated cells || IAPP
 +
|-
 +
| Rxn 6: || treated cells || KCNQ2
 +
|-
 +
| Rxn 7: || treated cells || GAPD (reference gene)
 +
|-
 +
| Rxn 8: || untreated cells || PDX1
 +
|-
 +
| Rxn 9: || untreated cells || MAFA
 +
|-
 +
| Rxn 10: || untreated cells || GLP1R
 +
|-
 +
| Rxn 11: || untreated cells || PCSK1
 +
|-
 +
| Rxn 12: || untreated cells || IAPP
 +
|-
 +
| Rxn 13: || untreated cells || KCNQ2
 +
|-
 +
| Rxn 14: || untreated cells || GAPD (reference gene)
 +
|-
 +
| Rxn 15: || no template || PDX1
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|-
 +
| Rxn 16: || no template || MAFA
 +
|-
 +
| Rxn 17: || no template || GLP1R
 +
|-
 +
| Rxn 18: || no template || PCSK1
 +
|-
 +
| Rxn 19: || no template || IAPP
 +
|-
 +
| Rxn 20: || no template || KCN2Q
 +
|-
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| Rxn 21: || no template || GAPD (reference gene)
 +
|}
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|}
|}
__NOTOC__
__NOTOC__

Revision as of 13:00, 4 November 2013

Haynes BioBrick Cloning Main project page
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UPLassay

  • This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.

Reaction List

REACTION LIST
  Template cDNA Gene Target
Rxn 1: treated cells PDX1
Rxn 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells IAPP
Rxn 6: treated cells KCNQ2
Rxn 7: treated cells GAPD (reference gene)
Rxn 8: untreated cells PDX1
Rxn 9: untreated cells MAFA
Rxn 10: untreated cells GLP1R
Rxn 11: untreated cells PCSK1
Rxn 12: untreated cells IAPP
Rxn 13: untreated cells KCNQ2
Rxn 14: untreated cells GAPD (reference gene)
Rxn 15: no template PDX1
Rxn 16: no template MAFA
Rxn 17: no template GLP1R
Rxn 18: no template PCSK1
Rxn 19: no template IAPP
Rxn 20: no template KCN2Q
Rxn 21: no template GAPD (reference gene)
  • Run 3 Each
  • 45 Reactions Run
  • Using Variation 2

Variation 2
Figure 1

  • Using as Reference, discount gene names on left side

Primers

PRIMERS LISt
  Roche Name Left Primer Right Primer
PDX1 Homo sapiens pancreatic and duodenal homeobox 1 (PDX1), mRNA NM_000209.3 aagctcacgcgtggaaag gccgtgagatgtacttgttgaa
Primer 2: treated cells MAFA
Rxn 3: treated cells GLP1R
Rxn 4: treated cells PCSK1
Rxn 5: treated cells IAPP
Rxn 6: treated cells KCNQ2
Rxn 7: treated cells GAPD (reference gene)
Rxn 8: untreated cells PDX1
Rxn 9: untreated cells MAFA
Rxn 10: untreated cells GLP1R
Rxn 11: untreated cells PCSK1
Rxn 12: untreated cells IAPP
Rxn 13: untreated cells KCNQ2
Rxn 14: untreated cells GAPD (reference gene)
Rxn 15: no template PDX1
Rxn 16: no template MAFA
Rxn 17: no template GLP1R
Rxn 18: no template PCSK1
Rxn 19: no template IAPP
Rxn 20: no template KCN2Q
Rxn 21: no template GAPD (reference gene)


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