UPLassay
- This is a test run for RT-PCR training. Using gene targets identified in prior research to pancreas differentiation gene sequences.
Reaction List
REACTION LIST
|
Template cDNA |
Gene Target
|
Rxn 1: |
treated cells |
PDX1
|
Rxn 2: |
treated cells |
MAFA
|
Rxn 3: |
treated cells |
GLP1R
|
Rxn 4: |
treated cells |
PCSK1
|
Rxn 5: |
treated cells |
IAPP
|
Rxn 6: |
treated cells |
KCNQ2
|
Rxn 7: |
treated cells |
GAPD (reference gene)
|
Rxn 8: |
untreated cells |
PDX1
|
Rxn 9: |
untreated cells |
MAFA
|
Rxn 10: |
untreated cells |
GLP1R
|
Rxn 11: |
untreated cells |
PCSK1
|
Rxn 12: |
untreated cells |
IAPP
|
Rxn 13: |
untreated cells |
KCNQ2
|
Rxn 14: |
untreated cells |
GAPD (reference gene)
|
Rxn 15: |
no template |
PDX1
|
Rxn 16: |
no template |
MAFA
|
Rxn 17: |
no template |
GLP1R
|
Rxn 18: |
no template |
PCSK1
|
Rxn 19: |
no template |
IAPP
|
Rxn 20: |
no template |
KCN2Q
|
Rxn 21: |
no template |
GAPD (reference gene)
|
- Run 3 Each
- 45 Reactions Run
- Using Variation 2
Variation 2
- Using as Reference, discount gene names on left side
Primers
PRIMERS LIST
|
Roche Name |
Left Primer |
Right Primer |
UPL probe
|
PDX1 |
NM_000209.3 |
gaaatccaccaaagctcacg |
cgggttccgctgtgtaag |
#51, cat.no. 04688481001
|
MAFA |
AB086961.1 |
ctccagagccaggtggag |
gtacaggtcccgctccttg |
#10, cat.no. 04685091001
|
GLP1R |
NM_021332.2 |
gatgggctcctctcctatca |
agatacacgccttccaccag |
#15, cat.no. 04685148001
|
PCSK1 |
NM_013628.2 |
tggagttgcatataattccaaagtt |
agcctcaatggcatcagttac |
#42, cat.no. 04688015001
|
IAPP |
NM_010491.2 |
aatgatgtgcatctccaaactg |
tggttcagtgccacagagag |
#101, cat.no. 04692195001
|
KCNQ2 |
NM_010611.2 |
cacgcctacgtgttcctttta |
cccctcagagctcttctcg |
#2, cat.no. 04684982001
|
GAPD (Ref) |
NM_008084.2 |
agcttgtcatcaacgggaag |
tttgatgttagtggggtctcg |
#9, cat.no. 04685075001
|
|
|
|