Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/07/22: Difference between revisions

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#Transforming Cells with plasmid DNA
==Transforming Cells with plasmid DNA==
#KAH126 MV2
#KAH126 MV2
#Ampicillin
#Ampicillin
Sample 1= KAH126
*Sample 1= KAH126
Sample 2 = Negative control.
*Sample 2 = Negative control.
Added 1 μL instead of 0.5μL to sample .
**Added 1 μL instead of 0.5μL to sample .
Incubated in ice for 10 min
**Incubated in ice for 10 min





Revision as of 13:57, 22 July 2015

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Transforming Cells with plasmid DNA

  1. KAH126 MV2
  2. Ampicillin
  • Sample 1= KAH126
  • Sample 2 = Negative control.
    • Added 1 μL instead of 0.5μL to sample .
    • Incubated in ice for 10 min