Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/07/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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*Electrophoresis
*Electrophoresis
**1kbt ladder - 10μL
**1kbt ladder - 10μL
**Sample 1 - 15μL
**Sample 1 KAH126/MV2 - 15μL
**Sample 2 - 15μL
**Sample 2 KAH126/MV2- 15μL
**Gel ran for 45 min
**Gel ran for 45 min
  [[Image:72815gel.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
  [[Image:72815gel.jpg|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]

Latest revision as of 01:04, 27 September 2017

Haynes BioBrick Cloning Main project page
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Gel Electrophoresis

  • 1% Agrose gel
    • 6μL sybr gel stain
  • Digests
    • DNA - 5.0μL
    • 10x FD buffer - 1.5μL
    • ECO RI - 1.0μL
    • Pst I - 1.0μL
    • dH2O - 6.5μL
  • Sample 1: DNA from colony 1
  • Sample 2: DNA from colony 2
    • Incubated at 37°C for 15 min
  • Electrophoresis
    • 1kbt ladder - 10μL
    • Sample 1 KAH126/MV2 - 15μL
    • Sample 2 KAH126/MV2- 15μL
    • Gel ran for 45 min
Today's gel
15 μL/lane; 1% agarose; Ladder
  • Two Bands in each sample
    • First bands approx 4500bp
    • Second bands approx 2000bp



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