Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/07/28: Difference between revisions

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(Autocreate 2015/07/28 Entry for Haynes_Lab:Notebook/David_Barclay_Undergrad_Training/Plasmid_Transformation)
 
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==Gel Electrophoresis==
*1% Agrose gel
**6μL sybr gel stain
*Digests
**DNA - 5.0μL
**10x FD buffer - 1.5μL
**ECO RI - 1.0μL
**Pst I - 1.0μL
**dH2O - 6.5μL
*Sample 1: DNA from colony 1
*Sample 2: DNA from colony 2
**Incubated at 37°C for 15 min
*Electrophoresis
**1kbt ladder - 10μL
**Sample 1 - 15μL
**Sample 2 - 15μL
**Gel ran for 45 min
[[Image:IMG 1099.PNG|400px|Today's gel]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]




Revision as of 15:37, 28 July 2015

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Gel Electrophoresis

  • 1% Agrose gel
    • 6μL sybr gel stain
  • Digests
    • DNA - 5.0μL
    • 10x FD buffer - 1.5μL
    • ECO RI - 1.0μL
    • Pst I - 1.0μL
    • dH2O - 6.5μL
  • Sample 1: DNA from colony 1
  • Sample 2: DNA from colony 2
    • Incubated at 37°C for 15 min
  • Electrophoresis
    • 1kbt ladder - 10μL
    • Sample 1 - 15μL
    • Sample 2 - 15μL
    • Gel ran for 45 min
Today's gel
15 μL/lane; 1% agarose; Ladder



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