Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/2015/08/02: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Haynes BioBrick Cloning</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Transforming Cells with Plasmid DNA==
==Transforming Cells with Plasmid DNA==
*B0004 MV2 - 1μL
*BD004/MV2 - 1μL
**1 negative control
**1 negative control
*B0006 MV2 - 1μL
*BD006/MV2 - 1μL
**1 negative control
**1 negative control
*Ampicillin
*Ampicillin
*Sample 1-4 = B0004 MV2
*Sample 1-4 = BD004/MV2
*Sample 2-4 = Negative Control (No B0004)
*Sample 2-4 = Negative Control (No B0004)
*Sample 1-6 = B0006 MV2
*Sample 1-6 = BD006/MV2
*Sample 2-6 = Negative Control (No B0006)
*Sample 2-6 = Negative Control (No B0006)
*10 min incubation in ice
*10 min incubation in ice

Latest revision as of 01:04, 27 September 2017

Haynes BioBrick Cloning Main project page
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Transforming Cells with Plasmid DNA

  • BD004/MV2 - 1μL
    • 1 negative control
  • BD006/MV2 - 1μL
    • 1 negative control
  • Ampicillin
  • Sample 1-4 = BD004/MV2
  • Sample 2-4 = Negative Control (No B0004)
  • Sample 1-6 = BD006/MV2
  • Sample 2-6 = Negative Control (No B0006)
  • 10 min incubation in ice




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