Haynes Lab:Notebook/David Barclay Undergrad Training/Plasmid Transformation/Entry Base: Difference between revisions
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====Causes of Errors==== | ====Causes of Errors==== | ||
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*Not exactly 600 μL of initial LB+sample. +-50 μL. '''Step A.2 | *Not exactly 600 μL of initial LB+sample. +-50 μL. '''[[Step A.2]]''' <br> | ||
*Pipette did not grab exactly 2 μL to add to Take 3 Plate. '''Step B.2''' <br> | *Pipette did not grab exactly 2 μL to add to Take 3 Plate. '''Step B.2''' <br> | ||
*Buffer used was Zyppy, not Water. Take 3 Plate could not have compensated. ''' Step B.3''' <br> | *Buffer used was Zyppy, not Water. Take 3 Plate could not have compensated. ''' Step B.3''' <br> |
Revision as of 12:56, 30 June 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
Tuesday, June 25thSteps for TransformationUsing KAH06 for Plasmid. Notes
Friday, June 28thMiniprepAims: Run plasmids through miniprep in order to isolate plasmid DNA and determine concentration. Protocol: A. Pre-Miniprep1. Add 3 mL LB into 15 mL tube B. Miniprep1. Pipette 1.5 mL of sample into 2 mL tube. Spin for 3 min @ max setting (13.1g). Repeat once more to spin entire sample. C. DNA Concentration- Take 3 Plate1. Open program Gen 5 2.0 before machine. Results
Causes of Errors
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