Haynes Lab:Notebook/Engineering PC-TFs/2012/02/16: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 22: Line 22:
* Place plate in EPOCH plate reader.
* Place plate in EPOCH plate reader.


Molarity :
Results:
 
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''1. KAH01    96.477  ng/µL'''
| align="center" style="background:#f0f0f0;"|'''Molarity of DNA Fragments'''
|-
| 1. KAH01    96.477  ng/µL
|-
|-
| 2. KAH03    122.149  ng/µL
| 2. KAH03    122.149  ng/µL
Line 38: Line 39:
|-
|-
| 7. KAH225  66.047  ng/µL
| 7. KAH225  66.047  ng/µL
 
|}
 
 


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 19:17, 17 February 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Ligation/Assembly

  • Weight gel slices.
  • Add three times amount of volume to each tube of gel (600 µL)
  • Incubate at 55°C for 5-10 minutes.
  • Centrifuge for a minute at 17,000 x g.
  • Add 20 µL of wash buffer.
  • Don't dump supernatent and centrifuge for one minute again.
  • Place spin columns in 1.5 mL tubes.

Measure concentration of each fragment:

  • Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application
  • Go to Nucleic Acid Quantification.
  • Pipette 2 µL of each DNA sequence into Take 3 plate.
  • Place plate in EPOCH plate reader.

Results:

Molarity of DNA Fragments
1. KAH01 96.477 ng/µL
2. KAH03 122.149 ng/µL
3. KAH04 80.182 ng/µL
4. KAH204 27.054 ng/µL
5. KAH205 47.105 ng/µL
6. KAH206 45.217 ng/µL
7. KAH225 66.047 ng/µL


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Engineering_PC-TFs/2012/02/16&oldid=585810"