Haynes Lab:Notebook/Engineering PC-TFs/2012/02/16: Difference between revisions
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*For all ligations, 25 ng of DNA vector = .3 µL | *For all ligations, 25 ng of DNA vector = .3 µL | ||
*There is a 2X molar ratio of insert:vector | *There is a 2X molar ratio of insert:vector | ||
<math> µL of insert = (length insert/lengthvector *2*25 ng vector/concentration insert) </math> | |||
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Revision as of 19:20, 17 February 2012
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||
Ligation/Assembly
Measure concentration of each fragment:
Results:
[math]\displaystyle{ µL of insert = (length insert/lengthvector *2*25 ng vector/concentration insert) }[/math] |