Haynes Lab:Notebook/Engineering PC-TFs/2012/02/16: Difference between revisions
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* Once completed, allow them to incubate for 10 minutes. | * Once completed, allow them to incubate for 10 minutes. | ||
* Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes. | * Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes. | ||
* Then follow transformation process | * Then follow transformation process: | ||
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Revision as of 19:39, 17 February 2012
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Ligation/Assembly
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Assembly Scheme: Number beside vector and insert is number of base pairs
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