Haynes Lab:Notebook/Engineering PC-TFs/2012/02/16: Difference between revisions
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|align="center" style="background:#f0f0f1;"|'''Transformation Process''' | |align="center" style="background:#f0f0f1;"|'''Transformation Process''' | ||
*Warm | *Warm 100 μg/mL Amp agar plates at 37 °C | ||
*Thaw fresh tube of DH5α Turbo cells on ice | *Thaw fresh tube of DH5α Turbo cells on ice | ||
*Add 30 μL of DH5α Turbo cells to DNA. | |||
*Add 30 μL of DH5α Turbo cells to DNA | |||
*Incubate cells + DNA on ice for 5 min. | *Incubate cells + DNA on ice for 5 min. | ||
*Label pre-warmed plates | *Label pre-warmed plates |
Revision as of 08:48, 6 September 2012
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Ligation/Assembly
Measure concentration of each fragment:
Results:
Assembly Scheme: Number beside vector and insert is number of base pairs
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