Haynes Lab:Notebook/Engineering PC-TFs/2012/03/01: Difference between revisions

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Assembly Scheme:
Assembly Scheme:
Number beside vector and insert is number of base pairs
Number beside vector and insert is number of base pairs
1. fshPCD (3386) + KAH204 (2328)
*1. fshPCD (3386) + KAH204 (2328)
2. fshPCD (3386) + KAH205 (1251)
*2. fshPCD (3386) + KAH205 (1251)
3. fshPCD (3386) + KAH206 (1551)
*3. fshPCD (3386) + KAH206 (1551)
4. fshPCD (3386) + KAH225 (1251)
*4. fshPCD (3386) + KAH225 (1251)
5. fshPCD (3386) only
*5. fshPCD (3386) only




Revision as of 15:37, 1 March 2012

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

Assembly Scheme: Number beside vector and insert is number of base pairs

  • 1. fshPCD (3386) + KAH204 (2328)
  • 2. fshPCD (3386) + KAH205 (1251)
  • 3. fshPCD (3386) + KAH206 (1551)
  • 4. fshPCD (3386) + KAH225 (1251)
  • 5. fshPCD (3386) only


' 1 2 3 4 5
DNA Insert 1.3 0.4 0.5 0.3 -----------
DNA Vector 0.3 0.3 0.3 0.3 0.3
T4 Ligase 1 1 1 1 1
Lign Buffer (2x) 5 5 5 5 5
dH2O 2.4 3.3 3.5 3.7 4
10 µL 10 µL 10 µL 10 µL 10 µL



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