Haynes Lab:Notebook/Engineering PC-TFs/2012/03/02: Difference between revisions

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=3/3/2012==
=3/3/2012==
* [[Image:ligationcheck.jpg|400px|thumb|right|Assembly Check for hPCD]]
[[Image:ligationcheck.jpg|400px|thumb|right|Assembly Check for hPCD]]


*1. hPCD (3386) + KAH204 (2328)= 5714
*1. hPCD (3386) + KAH204 (2328)= 5714
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* ==Results from Ligation for fshPCD==
*Results from Ligation for fshPCD
* There were zero colonies on negative control plate.
* There were zero colonies on negative control plate.
* For all ligation, > 10:1  
* For all ligation, > 10:1  

Revision as of 20:17, 3 March 2012

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3/3/2012=

Assembly Check for hPCD
  • 1. hPCD (3386) + KAH204 (2328)= 5714
  • 2. hPCD (3386) + KAH205 (1251)= 4637
  • 3. hPCD (3386) + KAH206 (1551)= 4937
  • 4. hPCD (3386) + KAH225 (1251)= 4637


  • Results from Ligation for fshPCD
  • There were zero colonies on negative control plate.
  • For all ligation, > 10:1